Evaluation of acridine Orange staining for rapid detection of mycobacteria in primary specimen
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چکیده
منابع مشابه
Acridine orange staining for rapid diagnosis of Acanthamoeba keratitis.
Acanthamoeba keratitis is uncommon, but one of the most severe infectious diseases of the cornea. Delayed diagnosis or misdiagnosis as bacterial or herpes simplex keratitis leads to extensive corneal inflammation and profound visual loss. Therefore, accurate and rapid diagnosis of Acanthamoeba keratitis is essential for successful treatment and good prognosis. We evaluated the usefulness of acr...
متن کاملUse of acridine orange staining for the detection of rotavirus RNA in polyacrylamide gels.
Acridine orange is a metachromatic intercalator used extensively in histochemistry to differentiate double- from single-stranded (ds, ss) nucleic acid by the emission of green and red fluorescence, respectively, under ultraviolet light. In the present study we standardised a protocol in order to use acridine orange to detect rotavirus ds RNA in polyacrylamide gels and compared it to silver and ...
متن کاملEvaluation of a rapid fluorescent staining method for detection of mycobacteria in clinical specimens.
Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less time ( approximately 2 min) to perform. These results suggest that the rapid st...
متن کاملStaining with an Acridine Orange Derivative for the Detection of Mycoplasmas in Cell Cultures
• Fischer 0., Dagmar Zendulkova: Staining With an Acridine Orange Derivative for the Detection of Mycoplasmas in Cell Cultures. Acta vet. Brno, 62,1993: 49-53. 24 cell cultures were examined for the presence of mycoplasmas by fluorescence methods using 3-amino-6-methoxy-9-(2-hydroxyethylamino) acridine (AMHA) or bisbenzimide 33258 (Hoechst), and by culture in liquid media containing glucose or ...
متن کاملComparative study of subculture, Gram staining and acridine orange staining for early detection of positive blood cultures.
In view of the importance of a rapid aetiological diagnosis in septicaemia, we compared the results of subculture, Gram staining and acridine orange staining in the detection of positive blood cultures. The study was based on 1013 blood cultures of which 138 were positive by culture. The three techniques were applied 12 h after the specimen was taken in 210 instances, at 24 h in 540 instances a...
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ژورنال
عنوان ژورنال: Indian Journal of Pathology and Microbiology
سال: 2010
ISSN: 0377-4929
DOI: 10.4103/0377-4929.72048